100 Bible Verses About Separation

Bible verses about Separation

Understanding separation through scripture is vital for spiritual growth. The Bible highlights different types of separation: from sin, from the world, and even physical separation for spiritual pursuits. Exploring these verses reveals God's desire for us to be set apart for His holy purposes. It's not about isolation, but about aligning our hearts and minds with His will. Through studying separation, we gain discernment, learn to prioritize God's kingdom, and cultivate a deeper intimacy with Him. Embracing the scriptural understanding of separation empowers us to live lives that reflect His light and truth in a world often pulling us away from Him.

Featured Verse

Genesis 31:27 (KJV)

Wherefore didst thou flee away secretly, and steal away from me; and didst not tell me, that I might have sent thee away with mirth, and with songs, with tabret, and with harp?

Separation in the Bible: Find Strength & Guidance

And he rose up that night, and took his two wives, and his two womenservants, and his eleven sons, and passed over the ford Jabbok.

Genesis 32:22KJV

Now therefore, Tatnai, governor beyond the river, Shethar–boznai, and your companions the Apharsachites, which are beyond the river, be ye far from thence:

Ezra 6:6KJV

And it was told Laban on the third day that Jacob was fled.

Genesis 31:22KJV

And they said, Thy servants are twelve brethren, the sons of one man in the land of Canaan; and, behold, the youngest is this day with our father, and one is not.

Genesis 42:13KJV

Then David went over to the other side, and stood on the top of an hill afar off; a great space being between them:

1 Samuel 26:13KJV

And they sent away Rebekah their sister, and her nurse, and Abraham’s servant, and his men.

Genesis 24:59KJV

Wherefore I command thee, saying, Thou shalt separate three cities for thee.

Deuteronomy 19:7KJV

For whatsoever soul it be that shall not be afflicted in that same day, he shall be cut off from among his people.

Leviticus 23:29KJV

And ye shall be holy unto me: for I the Lord am holy, and have severed you from other people, that ye should be mine.

Leviticus 20:26KJV

Abram dwelled in the land of Canaan, and Lot dwelled in the cities of the plain, and pitched his tent toward Sodom.

Genesis 13:12KJV

All the hangings of the court round about were of fine twined linen.

Exodus 38:16KJV

And that ye may put difference between holy and unholy, and between unclean and clean;

Leviticus 10:10KJV

Wherefore didst thou flee away secretly, and steal away from me; and didst not tell me, that I might have sent thee away with mirth, and with songs, with tabret, and with harp?

Genesis 31:27KJV

And the little owl, and the cormorant, and the great owl,

Leviticus 11:17KJV

And his concubine played the whore against him, and went away from him unto her father’s house to Beth–lehem–judah, and was there four whole months.

Judges 19:2KJV

And if it be on her bed, or on any thing whereon she sitteth, when he toucheth it, he shall be unclean until the even.

Leviticus 15:23KJV

Then the priest shall wash his clothes, and he shall bathe his flesh in water, and afterward he shall come into the camp, and the priest shall be unclean until the even.

Numbers 19:7KJV

And hast not suffered me to kiss my sons and my daughters? thou hast now done foolishly in so doing.

Genesis 31:28KJV

Then said the king to Ittai the Gittite, Wherefore goest thou also with us? return to thy place, and abide with the king: for thou art a stranger, and also an exile.

2 Samuel 15:19KJV

Thus shalt thou do unto all the cities which are very far off from thee, which are not of the cities of these nations.

Deuteronomy 20:15KJV

Then Samuel told the people the manner of the kingdom, and wrote it in a book, and laid it up before the Lord. And Samuel sent all the people away, every man to his house.

1 Samuel 10:25KJV

For afore the harvest, when the bud is perfect, and the sour grape is ripening in the flower, he shall both cut off the sprigs with pruning hooks, and take away and cut down the branches.

Isaiah 18:5KJV

Every bed whereon she lieth all the days of her issue shall be unto her as the bed of her separation: and whatsoever she sitteth upon shall be unclean, as the uncleanness of her separation.

Leviticus 15:26KJV

And this is the law of the Nazarite, when the days of his separation are fulfilled: he shall be brought unto the door of the tabernacle of the congregation:

Numbers 6:13KJV

This is the law of the Nazarite who hath vowed, and of his offering unto the Lord for his separation, beside that that his hand shall get: according to the vow which he vowed, so he must do after the law of his separation.

Numbers 6:21KJV

And if he that hath the issue spit upon him that is clean; then he shall wash his clothes, and bathe himself in water, and be unclean until the even.

Leviticus 15:8KJV

And Saul took him that day, and would let him go no more home to his father’s house.

1 Samuel 18:2KJV

And every beast that parteth the hoof, and cleaveth the cleft into two claws, and cheweth the cud among the beasts, that ye shall eat.

Deuteronomy 14:6KJV

Oh that my people had hearkened unto me, and Israel had walked in my ways!

Psalms 81:13KJV

Then he called his servant that ministered unto him, and said, Put now this woman out from me, and bolt the door after her.

2 Samuel 13:17KJV

Then Jethro, Moses’ father in law, took Zipporah, Moses’ wife, after he had sent her back,

Exodus 18:2KJV

But if ye will not hearken unto us, to be circumcised; then will we take our daughter, and we will be gone.

Genesis 34:17KJV

And Moses alone shall come near the Lord: but they shall not come nigh; neither shall the people go up with him.

Exodus 24:2KJV

And Moses let his father in law depart; and he went his way into his own land.

Exodus 18:27KJV

For the goodman is not at home, he is gone a long journey:

Proverbs 7:19KJV

But the Levites after the tribe of their fathers were not numbered among them.

Numbers 1:47KJV

It is better to dwell in the corner of the housetop, than with a brawling woman and in a wide house.

Proverbs 25:24KJV

Seven days shall there be no leaven found in your houses: for whosoever eateth that which is leavened, even that soul shall be cut off from the congregation of Israel, whether he be a stranger, or born in the land.

Exodus 12:19KJV

And he set the one in Beth–el, and the other put he in Dan.

1 Kings 12:29KJV

And whosoever beareth ought of the carcase of them shall wash his clothes, and be unclean until the even.

Leviticus 11:25KJV

If any man take a wife, and go in unto her, and hate her,

Deuteronomy 22:13KJV

Lo, then would I wander far off, and remain in the wilderness. Selah.

Psalms 55:7KJV

The ungodly are not so: but are like the chaff which the wind driveth away.

Psalms 1:4KJV

I opened to my beloved; but my beloved had withdrawn himself, and was gone: my soul failed when he spake: I sought him, but I could not find him; I called him, but he gave me no answer.

Song of Solomon 5:6KJV

He hath put my brethren far from me, and mine acquaintance are verily estranged from me.

Job 19:13KJV

And he spake unto the congregation, saying, Depart, I pray you, from the tents of these wicked men, and touch nothing of theirs, lest ye be consumed in all their sins.

Numbers 16:26KJV

Ye shall therefore put difference between clean beasts and unclean, and between unclean fowls and clean: and ye shall not make your souls abominable by beast, or by fowl, or by any manner of living thing that creepeth on the ground, which I have separated from you as unclean.

Leviticus 20:25KJV

Whatsoever hath no fins nor scales in the waters, that shall be an abomination unto you.

Leviticus 11:12KJV

And the hare, because he cheweth the cud, but divideth not the hoof; he is unclean unto you.

Leviticus 11:6KJV

And Abraham set seven ewe lambs of the flock by themselves.

Genesis 21:28KJV

Woe is me, that I sojourn in Mesech, that I dwell in the tents of Kedar!

Psalms 120:5KJV

I have hated the congregation of evil doers; and will not sit with the wicked.

Psalms 26:5KJV

All the days of the vow of his separation there shall no razor come upon his head: until the days be fulfilled, in the which he separateth himself unto the Lord, he shall be holy, and shall let the locks of the hair of his head grow.

Numbers 6:5KJV

Moreover ye shall eat no manner of blood, whether it be of fowl or of beast, in any of your dwellings.

Leviticus 7:26KJV

As far as the east is from the west, so far hath he removed our transgressions from us.

Psalms 103:12KJV

All the days that he separateth himself unto the Lord he shall come at no dead body.

Numbers 6:6KJV

To teach when it is unclean, and when it is clean: this is the law of leprosy.

Leviticus 14:57KJV

And he shall cause the house to be scraped within round about, and they shall pour out the dust that they scrape off without the city into an unclean place:

Leviticus 14:41KJV

But Benjamin, Joseph’s brother, Jacob sent not with his brethren; for he said, Lest peradventure mischief befall him.

Genesis 42:4KJV

Whereas thou camest but yesterday, should I this day make thee go up and down with us? seeing I go whither I may, return thou, and take back thy brethren: mercy and truth be with thee.

2 Samuel 15:20KJV

It is better to dwell in a corner of the housetop, than with a brawling woman in a wide house.

Proverbs 21:9KJV

Then I separated twelve of the chief of the priests, Sherebiah, Hashabiah, and ten of their brethren with them,

Ezra 8:24KJV

Yea, mine own familiar friend, in whom I trusted, which did eat of my bread, hath lifted up his heel against me.

Psalms 41:9KJV

Meddle not with them; for I will not give you of their land, no, not so much as a foot breadth; because I have given mount Seir unto Esau for a possession.

Deuteronomy 2:5KJV

And I will sever in that day the land of Goshen, in which my people dwell, that no swarms of flies shall be there; to the end thou mayest know that I am the Lord in the midst of the earth.

Exodus 8:22KJV

Then will I cause to cease from the cities of Judah, and from the streets of Jerusalem, the voice of mirth, and the voice of gladness, the voice of the bridegroom, and the voice of the bride: for the land shall be desolate.

Jeremiah 7:34KJV

And he that toucheth the flesh of him that hath the issue shall wash his clothes, and bathe himself in water, and be unclean until the even.

Leviticus 15:7KJV

For from the top of the rocks I see him, and from the hills I behold him: lo, the people shall dwell alone, and shall not be reckoned among the nations.

Numbers 23:9KJV

And every thing that she lieth upon in her separation shall be unclean: every thing also that she sitteth upon shall be unclean.

Leviticus 15:20KJV

And Abraham rose up early in the morning, and took bread, and a bottle of water, and gave it unto Hagar, putting it on her shoulder, and the child, and sent her away: and she departed, and wandered in the wilderness of Beer–sheba.

Genesis 21:14KJV

So Esau returned that day on his way unto Seir.

Genesis 33:16KJV

The lot causeth contentions to cease, and parteth between the mighty.

Proverbs 18:18KJV

And Abimelech said unto Isaac, Go from us; for thou art much mightier than we.

Genesis 26:16KJV

And thou shalt hang up the vail under the taches, that thou mayest bring in thither within the vail the ark of the testimony: and the vail shall divide unto you between the holy place and the most holy.

Exodus 26:33KJV

All the days of his separation shall he eat nothing that is made of the vine tree, from the kernels even to the husk.

Numbers 6:4KJV

And Abimelech said unto Abraham, What mean these seven ewe lambs which thou hast set by themselves?

Genesis 21:29KJV

And it shall be, if thou have no delight in her, then thou shalt let her go whither she will; but thou shalt not sell her at all for money, thou shalt not make merchandise of her, because thou hast humbled her.

Deuteronomy 21:14KJV

I said, I shall not see the Lord, even the Lord, in the land of the living: I shall behold man no more with the inhabitants of the world.

Isaiah 38:11KJV

Thou shalt not eat it; thou shalt pour it upon the earth as water.

Deuteronomy 12:24KJV

Thou shalt not plow with an ox and an ass together.

Deuteronomy 22:10KJV

And every thing whereupon any part of their carcase falleth shall be unclean; whether it be oven, or ranges for pots, they shall be broken down: for they are unclean, and shall be unclean unto you.

Leviticus 11:35KJV

And he said unto them, Depart yet for three days, then come again to me. And the people departed.

1 Kings 12:5KJV

And the covering of rams’ skins dyed red, and the covering of badgers’ skins, and the vail of the covering,

Exodus 39:34KJV

And the priest shall take the sodden shoulder of the ram, and one unleavened cake out of the basket, and one unleavened wafer, and shall put them upon the hands of the Nazarite, after the hair of his separation is shaven:

Numbers 6:19KJV

They shall not dwell in thy land, lest they make thee sin against me: for if thou serve their gods, it will surely be a snare unto thee.

Exodus 23:33KJV

And if a woman have an issue, and her issue in her flesh be blood, she shall be put apart seven days: and whosoever toucheth her shall be unclean until the even.

Leviticus 15:19KJV

But Zerubbabel, and Jeshua, and the rest of the chief of the fathers of Israel, said unto them, Ye have nothing to do with us to build an house unto our God; but we ourselves together will build unto the Lord God of Israel, as king Cyrus the king of Persia hath commanded us.

Ezra 4:3KJV

And every creeping thing that creepeth upon the earth shall be an abomination; it shall not be eaten.

Leviticus 11:41KJV

I will set no wicked thing before mine eyes: I hate the work of them that turn aside; it shall not cleave to me.

Psalms 101:3KJV

And one of the sons of Joiada, the son of Eliashib the high priest, was son in law to Sanballat the Horonite: therefore I chased him from me.

Nehemiah 13:28KJV

And he set three days’ journey betwixt himself and Jacob: and Jacob fed the rest of Laban’s flocks.

Genesis 30:36KJV

And they heard the voice of the Lord God walking in the garden in the cool of the day: and Adam and his wife hid themselves from the presence of the Lord God amongst the trees of the garden.

Genesis 3:8KJV

And whosoever toucheth those things shall be unclean, and shall wash his clothes, and bathe himself in water, and be unclean until the even.

Leviticus 15:27KJV

If there be among you any man, that is not clean by reason of uncleanness that chanceth him by night, then shall he go abroad out of the camp, he shall not come within the camp:

Deuteronomy 23:10KJV

And the king went forth, and all the people after him, and tarried in a place that was far off.

2 Samuel 15:17KJV

These also shall be unclean unto you among the creeping things that creep upon the earth; the weasel, and the mouse, and the tortoise after his kind,

Leviticus 11:29KJV

Where thou diest, will I die, and there will I be buried: the Lord do so to me, and more also, if ought but death part thee and me.

Ruth 1:17KJV

And he delivered them into the hand of his servants, every drove by themselves; and said unto his servants, Pass over before me, and put a space betwixt drove and drove.

Genesis 32:16KJV

Which say, Stand by thyself, come not near to me; for I am holier than thou. These are a smoke in my nose, a fire that burneth all the day.

Isaiah 65:5KJV

But I have said unto you, Ye shall inherit their land, and I will give it unto you to possess it, a land that floweth with milk and honey: I am the Lord your God, which have separated you from other people.

Leviticus 20:24KJV

The Bible's verses on separation, as explored here, reveal a consistent call to distinguish between the sacred and the profane, righteousness and wickedness. From separating ourselves from harmful influences to maintaining purity in our thoughts and actions, these passages emphasize the importance of discerning God's will and living a life set apart for His purpose. This isn't about isolation or condemnation but about a conscious choice to align ourselves with God's character and avoid the corrupting influence of the world. May these verses prompt deeper reflection on our own lives, challenging us to identify areas where separation is needed and empowering us to pursue holiness. As we intentionally choose to separate from what hinders our relationship with God, we draw closer to Him and experience the abundant blessings of a life lived in obedience and devotion.